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UV Cuvette Holders & Accessories Transmission CharacteristicsLaboratory UV absorbance spectroscopy requires the transmission of UV energy through samples held in cuvettes. The effectiveness of Ocean Optics spectrometers and accessories to perform these measurements depends on all optical elements in the system. The transmission through each cuvette holder was tested by using a deuterium tungsten halogen light source coupled to a solarization-resistant fiber, and an S2000-UV-VIS spectrometer coupled to another solarization-resistant fiber. The reference spectra was the signal obtained by coupling the two fibers with a splice bushing. The sample spectra were obtained with the cuvette holder in-line between the fibers. The cuvette holders use a common single lens adjustable collimator to change the wide-angle acceptance cone of the optical fiber into a collimated beam ~5 mm diameter. This lens is in focus only for a single wavelength, so that the throughput of the system is wavelength-dependent. The magnitude of this effect is quite dramatic.
This adjustability gives the user complete control of their optical system. If the experiment calls for measurements in the ultraviolet, the cuvette holder can be optimized to more efficiently transmit ultraviolet light. If absolute absorbance measurements are required (i.e. for the measurement of molar extinction coefficients) the entire optical system will have to be calibrated to determine the mean free path length of the cuvette and the cuvette holder. This is accomplished by measuring the absorbance of standard solutions. CUV-UV-10
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